Atlas of Subcellular RNA Localization Revealed by APEX-Seq

https://www.cell.com/cell/fulltext/S0092-8674(19)30555-0

Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang HY, Ting AY

• The authors introduce the method APEX-seq, which is a method for whole-transcriptome spatial profiling in living cells. It is based on direct proximity labelling of RNA using the peroxidase enzyme APEX2. 
• The APEX protein may be localised to different cellular subcomponents, such as the nucleolus, nuclear pore, endoplasmic reticulum, nuclear lamina, outer mitochondrial membrane, and mitochondrial matrix. Once there, APEX biotinylates mRNAs and proteins, allowing mRNAs from the targeted region to be purified and sequenced through RNA-seq.