MITO-Tag Mice enable rapid isolation and multimodal profiling of mitochondria from specific cell types in vivo

Erol C. Bayraktar, Lou Baudrier, Ceren Özerdem, Caroline A. Lewis, Sze Ham Chan, Tenzin Kunchok, Monther Abu-Remaileh, Andrew L. Cangelosi, David M. Sabatini, Kıvanç Birsoy, and Walter W. Chen

https://www.pnas.org/content/116/1/303?ijkey=eedc3db1701d7607eb1389c8dc45fe6c9903294c&keytype2=tf_ipsecsha

• The authors establish MITO-Tag mice, which allow cell type specific isolation of mitochondria from different tissues. These mice express a mitochondrially-localised epitope, whose expression is driven by a Cre-recombinase. Therefore, mice engineered such that Cre-recombinase is under the control of a promoter which is active in a particular cell type, allows isolation of mitochondria from particular cell types via immunoprecipitation.
• Purified mitochondria can be subsequently analysed through e.g. proteomic, lipidomic, and metabolomic analyses. The authors demonstrate this for hepatocytes.