Hauke S.Hillen, Yaroslav I.Morozov, Azadeh Sarfallah, Dmitry Temiakov, Patrick Cramer
- Transcription of the mitochondrial DNA is a critical aspect of understanding mitochondrial physiology. Not only is this linked to the generation of protein, as per nuclear transcription, there is also a link with the actual replication of mtDNA. Many molecular players are involved in both of these processes. Yet, mitochondrial transcription is not well understood in its details.
- The authors report crystal structures of the protein responsible for mtDNA transcription (mtRNAP) when attached to mtDNA.
- TFAM tethers to mtRNAP to recruit the protein to the mtDNA promoter site.
- TFB2M induces structural changes in mtRNAP to enable it to open mtDNA