Tuesday, 26 September 2017

Live imaging reveals the dynamics and regulation of mitochondrial nucleoids during the cell cycle in Fucci2-HeLa cells

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595809/

Taeko Sasaki, Yoshikatsu Sato, Tetsuya Higashiyama, Narie Sasaki

  •  Authors investigate the dynamics of mitochondrial DNA replication through the cell cycle by labelling HeLa cells with Fucci markers (a fluorescent probe which changes color according to the cell cycle stage) and SYBR Green I (which stains mtDNA)
  • Observe that mitochondrial nucleoids often attach/detach from each other
  • Mitochondrial replication occurs throughout the cell cycle, but peaks during the S-phase

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